/*************************************************************************** ** ** 454 Life Sciences Corporation ** ** Software Release: 2.5.3 ** ** Base Call Metrics Results ** ** File Created: 2011/06/24 11:12:23 ** ***************************************************************************/ /* ** Run Conditions */ runConditions { runName = "R_2011_06_24_11_12_23_FLX05100626_Administrator_A_Johanet_062411"; analysisName = "D_2011_06_24_11_13_44_FLX05100626_fullProcessingAmplicons"; PTPBarCode = "748495"; numberOfRegions = 8; numberOfCycles = 200; } /* ** Basecall Results */ basecallResults { numReads = 1245727; aveLength = 259.513; stdDev = 86.504; } /* ** Region-Key Results */ regionKey { region = 1; key = ATG; numberReads = 19306; totalBases = 10082888; averageLength = 522.267,24.195; averageQuality = 37.309,5.389; lengthHistogram { lengthCount = 52,1; lengthCount = 55,1; lengthCount = 58,1; lengthCount = 61,1; lengthCount = 77,1; lengthCount = 79,1; lengthCount = 80,1; lengthCount = 91,1; lengthCount = 103,1; lengthCount = 105,1; lengthCount = 190,1; lengthCount = 212,1; lengthCount = 215,1; lengthCount = 247,1; lengthCount = 251,1; lengthCount = 258,1; lengthCount = 280,1; lengthCount = 287,1; lengthCount = 288,1; lengthCount = 289,1; lengthCount = 319,1; lengthCount = 325,1; lengthCount = 337,2; lengthCount = 344,2; lengthCount = 345,1; lengthCount = 355,1; lengthCount = 377,1; lengthCount = 385,2; lengthCount = 392,1; lengthCount = 393,1; lengthCount = 397,1; lengthCount = 399,1; lengthCount = 403,1; lengthCount = 404,1; lengthCount = 405,1; lengthCount = 408,1; lengthCount = 409,1; lengthCount = 411,1; lengthCount = 415,1; lengthCount = 420,1; lengthCount = 428,1; lengthCount = 429,1; lengthCount = 431,1; lengthCount = 433,1; lengthCount = 434,1; lengthCount = 440,1; lengthCount = 441,1; lengthCount = 443,1; lengthCount = 447,1; lengthCount = 448,1; lengthCount = 457,1; lengthCount = 458,2; lengthCount = 462,1; lengthCount = 465,1; lengthCount = 468,2; lengthCount = 469,2; lengthCount = 472,1; lengthCount = 474,1; lengthCount = 475,2; lengthCount = 476,1; lengthCount = 478,3; lengthCount = 479,3; lengthCount = 480,1; lengthCount = 481,2; lengthCount = 482,8; lengthCount = 483,3; lengthCount = 484,454; lengthCount = 485,32; lengthCount = 486,14; lengthCount = 487,62; lengthCount = 488,1534; lengthCount = 489,1708; lengthCount = 490,513; lengthCount = 491,142; lengthCount = 492,64; lengthCount = 493,39; lengthCount = 494,25; lengthCount = 495,21; lengthCount = 496,11; lengthCount = 497,9; lengthCount = 498,9; lengthCount = 499,2; lengthCount = 500,2; lengthCount = 501,4; lengthCount = 502,2; lengthCount = 503,1; lengthCount = 504,2; lengthCount = 505,1; lengthCount = 506,3; lengthCount = 507,1; lengthCount = 509,2; lengthCount = 510,4; lengthCount = 511,5; lengthCount = 515,1; lengthCount = 516,1; lengthCount = 517,3; lengthCount = 518,6; lengthCount = 519,7; lengthCount = 520,8; lengthCount = 521,16; lengthCount = 522,49; lengthCount = 523,98; lengthCount = 524,335; lengthCount = 525,1388; lengthCount = 526,2871; lengthCount = 527,280; lengthCount = 528,93; lengthCount = 529,53; lengthCount = 530,36; lengthCount = 531,27; lengthCount = 532,29; lengthCount = 533,18; lengthCount = 534,18; lengthCount = 535,56; lengthCount = 536,50; lengthCount = 537,482; lengthCount = 538,3430; lengthCount = 539,4373; lengthCount = 540,470; lengthCount = 541,139; lengthCount = 542,53; lengthCount = 543,40; lengthCount = 544,22; lengthCount = 545,21; lengthCount = 546,14; lengthCount = 547,5; lengthCount = 548,14; lengthCount = 549,6; lengthCount = 550,6; lengthCount = 551,10; lengthCount = 552,4; lengthCount = 553,6; lengthCount = 554,3; lengthCount = 555,3; lengthCount = 556,2; lengthCount = 557,1; lengthCount = 559,2; lengthCount = 560,1; lengthCount = 562,1; lengthCount = 567,1; lengthCount = 570,1; } qualityHistogram { qualityBinCount = 0,493; qualityBinCount = 8,5; qualityBinCount = 9,2; qualityBinCount = 11,30679; qualityBinCount = 12,16096; qualityBinCount = 13,20530; qualityBinCount = 14,17871; qualityBinCount = 15,22579; qualityBinCount = 16,22376; qualityBinCount = 17,30798; qualityBinCount = 18,30389; qualityBinCount = 19,51025; qualityBinCount = 20,30309; qualityBinCount = 21,37433; qualityBinCount = 22,61971; qualityBinCount = 23,44624; qualityBinCount = 24,46711; qualityBinCount = 25,77026; qualityBinCount = 26,68972; qualityBinCount = 27,138080; qualityBinCount = 28,74321; qualityBinCount = 29,77543; qualityBinCount = 30,164074; qualityBinCount = 31,201360; qualityBinCount = 32,186416; qualityBinCount = 33,81350; qualityBinCount = 34,241531; qualityBinCount = 35,154961; qualityBinCount = 36,706277; qualityBinCount = 37,66629; qualityBinCount = 38,156472; qualityBinCount = 39,531915; qualityBinCount = 40,6692070; } region = 1; key = CAT; numberReads = 6693; totalBases = 3470072; averageLength = 518.463,20.334; averageQuality = 32.094,9.229; lengthHistogram { lengthCount = 62,1; lengthCount = 78,1; lengthCount = 91,1; lengthCount = 175,1; lengthCount = 201,1; lengthCount = 217,1; lengthCount = 229,1; lengthCount = 236,1; lengthCount = 316,1; lengthCount = 336,1; lengthCount = 342,1; lengthCount = 346,1; lengthCount = 352,1; lengthCount = 353,1; lengthCount = 357,1; lengthCount = 373,1; lengthCount = 380,1; lengthCount = 388,1; lengthCount = 397,1; lengthCount = 398,1; lengthCount = 401,1; lengthCount = 415,1; lengthCount = 419,1; lengthCount = 458,1; lengthCount = 472,1; lengthCount = 473,1; lengthCount = 475,1; lengthCount = 483,2; lengthCount = 484,1; lengthCount = 485,1; lengthCount = 489,1; lengthCount = 490,2; lengthCount = 491,2; lengthCount = 492,2; lengthCount = 493,6; lengthCount = 494,3; lengthCount = 495,4; lengthCount = 496,7; lengthCount = 497,16; lengthCount = 498,59; lengthCount = 499,364; lengthCount = 500,968; lengthCount = 501,341; lengthCount = 502,174; lengthCount = 503,113; lengthCount = 504,71; lengthCount = 505,28; lengthCount = 506,16; lengthCount = 507,14; lengthCount = 508,10; lengthCount = 509,8; lengthCount = 510,7; lengthCount = 511,7; lengthCount = 512,9; lengthCount = 513,14; lengthCount = 514,15; lengthCount = 515,27; lengthCount = 516,49; lengthCount = 517,65; lengthCount = 518,82; lengthCount = 519,117; lengthCount = 520,136; lengthCount = 521,156; lengthCount = 522,198; lengthCount = 523,210; lengthCount = 524,204; lengthCount = 525,172; lengthCount = 526,164; lengthCount = 527,143; lengthCount = 528,142; lengthCount = 529,200; lengthCount = 530,259; lengthCount = 531,338; lengthCount = 532,429; lengthCount = 533,395; lengthCount = 534,291; lengthCount = 535,192; lengthCount = 536,119; lengthCount = 537,91; lengthCount = 538,53; lengthCount = 539,43; lengthCount = 540,37; lengthCount = 541,23; lengthCount = 542,15; lengthCount = 543,16; lengthCount = 544,12; lengthCount = 545,4; lengthCount = 546,4; lengthCount = 547,2; lengthCount = 548,2; lengthCount = 549,1; lengthCount = 550,3; lengthCount = 551,2; lengthCount = 552,2; lengthCount = 556,1; lengthCount = 561,1; lengthCount = 571,1; } qualityHistogram { qualityBinCount = 0,779; qualityBinCount = 8,330; qualityBinCount = 9,726; qualityBinCount = 10,17; qualityBinCount = 11,100948; qualityBinCount = 12,40045; qualityBinCount = 13,47515; qualityBinCount = 14,37420; qualityBinCount = 15,54350; qualityBinCount = 16,48133; qualityBinCount = 17,62046; qualityBinCount = 18,56997; qualityBinCount = 19,63589; qualityBinCount = 20,45032; qualityBinCount = 21,64612; qualityBinCount = 22,55204; qualityBinCount = 23,69453; qualityBinCount = 24,50468; qualityBinCount = 25,71908; qualityBinCount = 26,83199; qualityBinCount = 27,63666; qualityBinCount = 28,72294; qualityBinCount = 29,54301; qualityBinCount = 30,82041; qualityBinCount = 31,65117; qualityBinCount = 32,79719; qualityBinCount = 33,86665; qualityBinCount = 34,73535; qualityBinCount = 35,92184; qualityBinCount = 36,79479; qualityBinCount = 37,177864; qualityBinCount = 38,95340; qualityBinCount = 39,293335; qualityBinCount = 40,1201761; } region = 1; key = TCA; numberReads = 40647; totalBases = 9778761; averageLength = 240.578,55.306; averageQuality = 37.057,5.740; lengthHistogram { lengthCount = 44,1; lengthCount = 47,1; lengthCount = 48,2; lengthCount = 49,3; lengthCount = 50,14; lengthCount = 51,23; lengthCount = 52,104; lengthCount = 53,211; lengthCount = 54,38; lengthCount = 55,25; lengthCount = 56,19; lengthCount = 57,26; lengthCount = 58,39; lengthCount = 59,8; lengthCount = 60,47; lengthCount = 61,86; lengthCount = 62,82; lengthCount = 63,116; lengthCount = 64,31; lengthCount = 65,88; lengthCount = 66,16; lengthCount = 67,19; lengthCount = 68,15; lengthCount = 69,23; lengthCount = 70,24; lengthCount = 71,15; lengthCount = 72,15; lengthCount = 73,5; lengthCount = 74,45; lengthCount = 75,21; lengthCount = 76,128; lengthCount = 77,16; lengthCount = 78,18; lengthCount = 79,17; lengthCount = 80,13; lengthCount = 81,11; lengthCount = 82,8; lengthCount = 83,17; lengthCount = 84,5; lengthCount = 85,10; lengthCount = 86,39; lengthCount = 87,180; lengthCount = 88,42; lengthCount = 89,377; lengthCount = 90,30; lengthCount = 91,15; 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lengthCount = 379,6; lengthCount = 380,5; lengthCount = 381,4; lengthCount = 382,2; lengthCount = 383,3; lengthCount = 384,5; lengthCount = 385,3; lengthCount = 386,5; lengthCount = 387,1; lengthCount = 388,3; lengthCount = 389,3; lengthCount = 390,3; lengthCount = 393,3; lengthCount = 394,3; lengthCount = 395,7; lengthCount = 396,9; lengthCount = 397,1; lengthCount = 398,7; lengthCount = 399,2; lengthCount = 400,1; lengthCount = 403,3; lengthCount = 404,2; lengthCount = 405,1; lengthCount = 406,3; lengthCount = 407,1; lengthCount = 410,3; lengthCount = 411,1; lengthCount = 412,3; lengthCount = 413,6; lengthCount = 414,4; lengthCount = 415,1; lengthCount = 416,1; lengthCount = 418,1; lengthCount = 419,1; lengthCount = 420,1; lengthCount = 421,2; lengthCount = 422,1; lengthCount = 424,2; lengthCount = 425,1; lengthCount = 426,1; lengthCount = 429,4; lengthCount = 433,3; lengthCount = 435,2; lengthCount = 436,1; lengthCount = 437,1; lengthCount = 438,1; lengthCount = 441,1; lengthCount = 442,2; lengthCount = 443,1; lengthCount = 451,1; lengthCount = 452,2; lengthCount = 456,2; lengthCount = 457,1; lengthCount = 458,1; lengthCount = 459,1; lengthCount = 461,1; lengthCount = 462,2; lengthCount = 463,3; lengthCount = 464,1; lengthCount = 466,1; lengthCount = 467,1; lengthCount = 469,2; lengthCount = 471,1; lengthCount = 473,1; lengthCount = 474,3; lengthCount = 477,3; lengthCount = 479,3; lengthCount = 481,2; lengthCount = 484,2; lengthCount = 485,3; lengthCount = 486,2; lengthCount = 491,3; lengthCount = 492,2; lengthCount = 493,2; lengthCount = 494,1; lengthCount = 495,3; lengthCount = 496,1; lengthCount = 499,2; lengthCount = 501,4; lengthCount = 503,3; lengthCount = 504,3; lengthCount = 505,2; lengthCount = 506,2; lengthCount = 507,3; lengthCount = 508,4; lengthCount = 509,3; lengthCount = 510,2; lengthCount = 512,1; lengthCount = 513,1; lengthCount = 515,1; lengthCount = 517,1; lengthCount = 518,1; lengthCount = 519,1; lengthCount = 520,2; lengthCount = 521,2; lengthCount = 522,2; lengthCount = 523,1; lengthCount = 524,1; lengthCount = 525,1; lengthCount = 527,2; lengthCount = 528,1; lengthCount = 530,1; lengthCount = 531,1; lengthCount = 533,1; lengthCount = 538,1; lengthCount = 546,1; lengthCount = 550,1; lengthCount = 551,1; lengthCount = 553,1; lengthCount = 598,1; } qualityHistogram { qualityBinCount = 0,1000; qualityBinCount = 7,3; qualityBinCount = 8,33; qualityBinCount = 9,47; qualityBinCount = 10,219; qualityBinCount = 11,16347; qualityBinCount = 12,27223; qualityBinCount = 13,17212; qualityBinCount = 14,38118; qualityBinCount = 15,16047; qualityBinCount = 16,38947; qualityBinCount = 17,31212; qualityBinCount = 18,29491; qualityBinCount = 19,56515; qualityBinCount = 20,49451; qualityBinCount = 21,75352; qualityBinCount = 22,50584; qualityBinCount = 23,56593; qualityBinCount = 24,56183; qualityBinCount = 25,65005; qualityBinCount = 26,136229; qualityBinCount = 27,80638; qualityBinCount = 28,110203; qualityBinCount = 29,80938; qualityBinCount = 30,221564; qualityBinCount = 31,101438; qualityBinCount = 32,106039; qualityBinCount = 33,182050; qualityBinCount = 34,333516; qualityBinCount = 35,175056; qualityBinCount = 36,191607; qualityBinCount = 37,119815; qualityBinCount = 38,269884; qualityBinCount = 39,1022546; qualityBinCount = 40,6021656; } } regionKey { region = 2; key = ATG; numberReads = 3631; totalBases = 1894987; averageLength = 521.891,22.537; averageQuality = 36.267,6.398; lengthHistogram { lengthCount = 178,1; lengthCount = 185,1; lengthCount = 286,1; lengthCount = 289,1; lengthCount = 352,1; lengthCount = 353,1; lengthCount = 400,1; lengthCount = 464,1; lengthCount = 479,2; lengthCount = 480,1; lengthCount = 482,1; lengthCount = 483,2; lengthCount = 484,62; lengthCount = 485,3; lengthCount = 486,3; lengthCount = 487,1; lengthCount = 488,313; lengthCount = 489,255; lengthCount = 490,146; lengthCount = 491,40; lengthCount = 492,12; lengthCount = 493,6; lengthCount = 494,6; lengthCount = 495,5; lengthCount = 496,1; lengthCount = 497,5; lengthCount = 498,1; lengthCount = 499,1; lengthCount = 501,1; lengthCount = 502,2; lengthCount = 511,1; lengthCount = 513,2; lengthCount = 514,2; lengthCount = 516,2; lengthCount = 517,2; lengthCount = 518,1; lengthCount = 519,2; lengthCount = 520,4; lengthCount = 521,8; lengthCount = 522,24; lengthCount = 523,91; lengthCount = 524,614; lengthCount = 525,135; lengthCount = 526,53; lengthCount = 527,25; lengthCount = 528,20; lengthCount = 529,13; lengthCount = 530,8; lengthCount = 531,5; lengthCount = 532,7; lengthCount = 533,5; lengthCount = 534,5; lengthCount = 535,15; lengthCount = 536,23; lengthCount = 537,531; lengthCount = 538,767; lengthCount = 539,270; lengthCount = 540,61; lengthCount = 541,19; lengthCount = 542,13; lengthCount = 543,2; lengthCount = 544,6; lengthCount = 545,6; lengthCount = 546,3; lengthCount = 548,2; lengthCount = 550,1; lengthCount = 551,1; lengthCount = 552,2; lengthCount = 555,1; lengthCount = 556,1; lengthCount = 561,1; } qualityHistogram { qualityBinCount = 0,183; qualityBinCount = 8,1; qualityBinCount = 11,10087; qualityBinCount = 12,3866; qualityBinCount = 13,6481; qualityBinCount = 14,4449; qualityBinCount = 15,7372; qualityBinCount = 16,6043; qualityBinCount = 17,10062; qualityBinCount = 18,9437; qualityBinCount = 19,14716; qualityBinCount = 20,10003; qualityBinCount = 21,12233; qualityBinCount = 22,13914; qualityBinCount = 23,17912; qualityBinCount = 24,11612; qualityBinCount = 25,29462; qualityBinCount = 26,19424; qualityBinCount = 27,30389; qualityBinCount = 28,26525; qualityBinCount = 29,32069; qualityBinCount = 30,42748; qualityBinCount = 31,30231; qualityBinCount = 32,54965; qualityBinCount = 33,27410; qualityBinCount = 34,37266; qualityBinCount = 35,37849; qualityBinCount = 36,71168; qualityBinCount = 37,30589; qualityBinCount = 38,59612; qualityBinCount = 39,104988; qualityBinCount = 40,1121921; } region = 2; key = CAT; numberReads = 1004; totalBases = 515881; averageLength = 513.826,32.277; averageQuality = 30.215,9.506; lengthHistogram { lengthCount = 69,1; lengthCount = 111,1; lengthCount = 113,1; lengthCount = 218,1; lengthCount = 231,1; lengthCount = 290,1; lengthCount = 368,1; lengthCount = 379,1; lengthCount = 398,1; lengthCount = 416,1; lengthCount = 432,1; lengthCount = 489,1; lengthCount = 490,1; lengthCount = 491,2; lengthCount = 492,1; lengthCount = 493,4; lengthCount = 494,1; lengthCount = 495,2; lengthCount = 496,2; lengthCount = 497,11; lengthCount = 498,40; lengthCount = 499,101; lengthCount = 500,93; lengthCount = 501,78; lengthCount = 502,33; lengthCount = 503,23; lengthCount = 504,13; lengthCount = 505,9; lengthCount = 506,7; lengthCount = 507,8; lengthCount = 508,2; lengthCount = 509,4; lengthCount = 510,3; lengthCount = 511,1; lengthCount = 512,2; lengthCount = 513,3; lengthCount = 514,2; lengthCount = 515,2; lengthCount = 516,3; lengthCount = 517,6; lengthCount = 518,8; lengthCount = 519,10; lengthCount = 520,15; lengthCount = 521,18; lengthCount = 522,23; lengthCount = 523,25; lengthCount = 524,22; lengthCount = 525,18; lengthCount = 526,35; lengthCount = 527,23; lengthCount = 528,36; lengthCount = 529,38; lengthCount = 530,29; lengthCount = 531,29; lengthCount = 532,34; lengthCount = 533,39; lengthCount = 534,19; lengthCount = 535,24; lengthCount = 536,17; lengthCount = 537,19; lengthCount = 538,9; lengthCount = 539,10; lengthCount = 540,4; lengthCount = 541,8; lengthCount = 542,8; lengthCount = 543,5; lengthCount = 544,3; lengthCount = 545,2; lengthCount = 546,2; lengthCount = 548,1; lengthCount = 549,1; lengthCount = 558,1; } qualityHistogram { qualityBinCount = 0,136; qualityBinCount = 8,81; qualityBinCount = 9,137; qualityBinCount = 10,4; qualityBinCount = 11,21462; qualityBinCount = 12,7679; qualityBinCount = 13,9655; qualityBinCount = 14,5771; qualityBinCount = 15,12386; qualityBinCount = 16,8007; qualityBinCount = 17,11763; qualityBinCount = 18,10160; qualityBinCount = 19,11912; qualityBinCount = 20,6389; qualityBinCount = 21,11625; qualityBinCount = 22,9458; qualityBinCount = 23,13733; qualityBinCount = 24,9384; qualityBinCount = 25,12554; qualityBinCount = 26,12653; qualityBinCount = 27,11092; qualityBinCount = 28,13323; qualityBinCount = 29,8754; qualityBinCount = 30,14240; qualityBinCount = 31,9160; qualityBinCount = 32,15223; qualityBinCount = 33,16295; qualityBinCount = 34,7317; qualityBinCount = 35,22652; qualityBinCount = 36,7549; qualityBinCount = 37,54318; qualityBinCount = 38,13409; qualityBinCount = 39,34989; qualityBinCount = 40,112611; } region = 2; key = TCA; numberReads = 74556; totalBases = 17653011; averageLength = 236.775,54.952; averageQuality = 37.126,5.734; lengthHistogram { lengthCount = 44,1; lengthCount = 46,1; lengthCount = 47,1; lengthCount = 48,3; lengthCount = 49,4; lengthCount = 50,10; lengthCount = 51,24; lengthCount = 52,91; lengthCount = 53,186; lengthCount = 54,46; lengthCount = 55,34; lengthCount = 56,26; lengthCount = 57,33; lengthCount = 58,42; lengthCount = 59,24; lengthCount = 60,52; lengthCount = 61,145; lengthCount = 62,172; lengthCount = 63,356; lengthCount = 64,58; 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lengthCount = 498,3; lengthCount = 499,5; lengthCount = 500,5; lengthCount = 502,4; lengthCount = 503,1; lengthCount = 505,5; lengthCount = 506,2; lengthCount = 507,2; lengthCount = 508,1; lengthCount = 510,1; lengthCount = 511,4; lengthCount = 515,2; lengthCount = 516,1; lengthCount = 517,2; lengthCount = 518,1; lengthCount = 520,5; lengthCount = 521,1; lengthCount = 522,4; lengthCount = 523,1; lengthCount = 524,2; lengthCount = 525,5; lengthCount = 526,2; lengthCount = 527,3; lengthCount = 529,3; lengthCount = 534,1; lengthCount = 535,1; lengthCount = 536,1; lengthCount = 538,2; lengthCount = 540,3; lengthCount = 541,2; lengthCount = 544,1; lengthCount = 545,1; lengthCount = 551,1; lengthCount = 555,1; lengthCount = 578,1; lengthCount = 584,1; lengthCount = 616,1; lengthCount = 649,1; lengthCount = 666,1; } qualityHistogram { qualityBinCount = 0,1299; qualityBinCount = 7,12; qualityBinCount = 8,100; qualityBinCount = 9,123; qualityBinCount = 10,637; qualityBinCount = 11,34303; qualityBinCount = 12,52356; qualityBinCount = 13,33763; qualityBinCount = 14,67689; qualityBinCount = 15,31421; qualityBinCount = 16,70826; qualityBinCount = 17,61061; qualityBinCount = 18,52277; qualityBinCount = 19,101601; qualityBinCount = 20,87521; qualityBinCount = 21,132025; qualityBinCount = 22,89070; qualityBinCount = 23,94067; qualityBinCount = 24,96367; qualityBinCount = 25,112995; qualityBinCount = 26,230116; qualityBinCount = 27,132845; qualityBinCount = 28,183165; qualityBinCount = 29,143506; qualityBinCount = 30,374969; qualityBinCount = 31,180398; qualityBinCount = 32,185914; qualityBinCount = 33,319898; qualityBinCount = 34,631536; qualityBinCount = 35,281117; qualityBinCount = 36,313296; qualityBinCount = 37,239254; qualityBinCount = 38,442443; qualityBinCount = 39,1773146; qualityBinCount = 40,11101895; } } regionKey { region = 3; key = ATG; numberReads = 1079; totalBases = 562570; averageLength = 521.381,25.331; averageQuality = 34.182,7.487; lengthHistogram { lengthCount = 132,1; lengthCount = 149,1; lengthCount = 476,1; lengthCount = 478,1; lengthCount = 483,1; lengthCount = 484,14; lengthCount = 485,2; lengthCount = 486,3; lengthCount = 487,5; lengthCount = 488,47; lengthCount = 489,56; lengthCount = 490,58; lengthCount = 491,35; lengthCount = 492,18; lengthCount = 493,7; lengthCount = 494,5; lengthCount = 495,1; lengthCount = 505,1; lengthCount = 515,1; lengthCount = 516,1; lengthCount = 519,1; lengthCount = 520,1; lengthCount = 521,13; lengthCount = 522,53; lengthCount = 523,91; lengthCount = 524,98; lengthCount = 525,35; lengthCount = 526,9; lengthCount = 527,1; lengthCount = 528,2; lengthCount = 530,1; lengthCount = 531,1; lengthCount = 532,2; lengthCount = 533,5; lengthCount = 534,10; lengthCount = 535,27; lengthCount = 536,65; lengthCount = 537,136; lengthCount = 538,165; lengthCount = 539,71; lengthCount = 540,20; lengthCount = 541,4; lengthCount = 542,5; lengthCount = 544,1; lengthCount = 552,1; lengthCount = 553,1; lengthCount = 556,1; } qualityHistogram { qualityBinCount = 0,84; qualityBinCount = 11,3212; qualityBinCount = 12,2850; qualityBinCount = 13,3274; qualityBinCount = 14,1919; qualityBinCount = 15,3987; qualityBinCount = 16,3164; qualityBinCount = 17,5784; qualityBinCount = 18,5137; qualityBinCount = 19,7604; qualityBinCount = 20,4325; qualityBinCount = 21,7609; qualityBinCount = 22,5758; qualityBinCount = 23,11921; qualityBinCount = 24,6550; qualityBinCount = 25,15071; qualityBinCount = 26,10607; qualityBinCount = 27,13379; qualityBinCount = 28,14092; qualityBinCount = 29,13925; qualityBinCount = 30,16159; qualityBinCount = 31,9313; qualityBinCount = 32,18139; qualityBinCount = 33,17171; qualityBinCount = 34,7666; qualityBinCount = 35,20774; qualityBinCount = 36,12311; qualityBinCount = 37,21233; qualityBinCount = 38,26353; qualityBinCount = 39,37820; qualityBinCount = 40,235379; } region = 3; key = CAT; numberReads = 207; totalBases = 106474; averageLength = 514.367,20.292; averageQuality = 27.812,8.959; lengthHistogram { lengthCount = 307,1; lengthCount = 477,1; lengthCount = 487,1; lengthCount = 495,1; lengthCount = 497,2; lengthCount = 498,11; lengthCount = 499,6; lengthCount = 500,8; lengthCount = 501,12; lengthCount = 502,12; lengthCount = 503,13; lengthCount = 504,9; lengthCount = 505,10; lengthCount = 506,6; lengthCount = 507,4; lengthCount = 508,2; lengthCount = 509,3; lengthCount = 510,2; lengthCount = 511,1; lengthCount = 514,1; lengthCount = 516,1; lengthCount = 517,1; lengthCount = 518,2; lengthCount = 519,3; lengthCount = 520,1; lengthCount = 521,4; lengthCount = 522,3; lengthCount = 523,2; lengthCount = 524,3; lengthCount = 525,4; lengthCount = 526,8; lengthCount = 527,8; lengthCount = 528,6; lengthCount = 529,6; lengthCount = 530,5; lengthCount = 531,7; lengthCount = 532,7; lengthCount = 533,8; lengthCount = 534,8; lengthCount = 535,3; lengthCount = 536,3; lengthCount = 537,3; lengthCount = 538,3; lengthCount = 542,2; } qualityHistogram { qualityBinCount = 0,58; qualityBinCount = 8,7; qualityBinCount = 9,7; qualityBinCount = 10,3; qualityBinCount = 11,4676; qualityBinCount = 12,1892; qualityBinCount = 13,2607; qualityBinCount = 14,1092; qualityBinCount = 15,3108; qualityBinCount = 16,1646; qualityBinCount = 17,3130; qualityBinCount = 18,2564; qualityBinCount = 19,3471; qualityBinCount = 20,1300; qualityBinCount = 21,3194; qualityBinCount = 22,2685; qualityBinCount = 23,4255; qualityBinCount = 24,2848; qualityBinCount = 25,3917; qualityBinCount = 26,2953; qualityBinCount = 27,3887; qualityBinCount = 28,3862; qualityBinCount = 29,2375; qualityBinCount = 30,3995; qualityBinCount = 31,2299; qualityBinCount = 32,4556; qualityBinCount = 33,4336; qualityBinCount = 34,1189; qualityBinCount = 35,5961; qualityBinCount = 36,1206; qualityBinCount = 37,11798; qualityBinCount = 38,1577; qualityBinCount = 39,5250; qualityBinCount = 40,8770; } region = 3; key = TCA; numberReads = 80582; totalBases = 19598909; averageLength = 243.217,55.281; averageQuality = 36.748,5.980; lengthHistogram { lengthCount = 44,1; lengthCount = 45,2; 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lengthCount = 533,1; lengthCount = 534,2; lengthCount = 536,1; lengthCount = 537,2; lengthCount = 538,2; lengthCount = 539,1; lengthCount = 540,1; lengthCount = 542,1; lengthCount = 543,1; lengthCount = 545,1; lengthCount = 548,1; lengthCount = 549,1; lengthCount = 565,1; lengthCount = 630,1; } qualityHistogram { qualityBinCount = 0,1689; qualityBinCount = 7,10; qualityBinCount = 8,77; qualityBinCount = 9,170; qualityBinCount = 10,762; qualityBinCount = 11,45141; qualityBinCount = 12,61194; qualityBinCount = 13,37312; qualityBinCount = 14,81491; qualityBinCount = 15,34213; qualityBinCount = 16,86882; qualityBinCount = 17,69710; qualityBinCount = 18,65637; qualityBinCount = 19,135155; qualityBinCount = 20,106186; qualityBinCount = 21,163903; qualityBinCount = 22,109662; qualityBinCount = 23,142561; qualityBinCount = 24,127779; qualityBinCount = 25,151554; qualityBinCount = 26,261936; qualityBinCount = 27,197371; qualityBinCount = 28,250756; qualityBinCount = 29,184567; qualityBinCount = 30,431030; qualityBinCount = 31,234259; qualityBinCount = 32,260233; qualityBinCount = 33,448539; qualityBinCount = 34,647077; qualityBinCount = 35,401333; qualityBinCount = 36,397024; qualityBinCount = 37,353461; qualityBinCount = 38,661488; qualityBinCount = 39,1927870; qualityBinCount = 40,11520877; } } regionKey { region = 4; key = ATG; numberReads = 704; totalBases = 366552; averageLength = 520.670,26.719; averageQuality = 33.516,7.959; lengthHistogram { lengthCount = 152,1; lengthCount = 198,1; lengthCount = 484,5; lengthCount = 485,3; lengthCount = 486,3; lengthCount = 487,15; lengthCount = 488,50; lengthCount = 489,41; lengthCount = 490,25; lengthCount = 491,12; lengthCount = 492,4; lengthCount = 493,2; lengthCount = 494,3; lengthCount = 497,1; lengthCount = 499,1; lengthCount = 500,2; lengthCount = 506,1; lengthCount = 515,1; lengthCount = 519,2; lengthCount = 520,5; lengthCount = 521,7; lengthCount = 522,36; lengthCount = 523,71; lengthCount = 524,40; lengthCount = 525,25; lengthCount = 526,10; lengthCount = 527,4; lengthCount = 528,1; lengthCount = 529,3; lengthCount = 530,1; lengthCount = 531,3; lengthCount = 532,4; lengthCount = 533,3; lengthCount = 534,7; lengthCount = 535,22; lengthCount = 536,69; lengthCount = 537,87; lengthCount = 538,68; lengthCount = 539,46; lengthCount = 540,10; lengthCount = 541,2; lengthCount = 542,3; lengthCount = 544,1; lengthCount = 545,1; lengthCount = 546,1; lengthCount = 560,1; } qualityHistogram { qualityBinCount = 0,23; qualityBinCount = 11,3801; qualityBinCount = 12,2142; qualityBinCount = 13,2606; qualityBinCount = 14,1365; qualityBinCount = 15,3845; qualityBinCount = 16,2525; qualityBinCount = 17,4786; qualityBinCount = 18,3933; qualityBinCount = 19,6124; qualityBinCount = 20,3117; qualityBinCount = 21,6050; qualityBinCount = 22,4421; qualityBinCount = 23,8967; qualityBinCount = 24,4738; qualityBinCount = 25,10608; qualityBinCount = 26,6875; qualityBinCount = 27,9051; qualityBinCount = 28,8843; qualityBinCount = 29,8106; qualityBinCount = 30,10392; qualityBinCount = 31,6219; qualityBinCount = 32,11779; qualityBinCount = 33,11173; qualityBinCount = 34,4919; qualityBinCount = 35,13755; qualityBinCount = 36,7301; qualityBinCount = 37,16661; qualityBinCount = 38,15084; qualityBinCount = 39,24374; qualityBinCount = 40,142969; } region = 4; key = CAT; numberReads = 132; totalBases = 66666; averageLength = 505.045,39.630; averageQuality = 28.315,9.090; lengthHistogram { lengthCount = 119,1; lengthCount = 322,1; lengthCount = 493,1; lengthCount = 495,2; lengthCount = 496,1; lengthCount = 497,7; lengthCount = 498,10; lengthCount = 499,17; lengthCount = 500,11; lengthCount = 501,8; lengthCount = 502,6; lengthCount = 503,5; lengthCount = 504,2; lengthCount = 505,6; lengthCount = 506,4; lengthCount = 507,2; lengthCount = 509,3; lengthCount = 510,1; lengthCount = 513,1; lengthCount = 515,1; lengthCount = 516,1; lengthCount = 518,3; lengthCount = 519,1; lengthCount = 520,2; lengthCount = 521,3; lengthCount = 523,1; lengthCount = 524,3; lengthCount = 525,3; lengthCount = 526,4; lengthCount = 527,3; lengthCount = 528,3; lengthCount = 529,5; lengthCount = 531,3; lengthCount = 532,3; lengthCount = 533,2; lengthCount = 538,1; lengthCount = 559,1; } qualityHistogram { qualityBinCount = 0,13; qualityBinCount = 8,8; qualityBinCount = 9,23; qualityBinCount = 11,2816; qualityBinCount = 12,969; qualityBinCount = 13,1577; qualityBinCount = 14,706; qualityBinCount = 15,1973; qualityBinCount = 16,884; qualityBinCount = 17,2151; qualityBinCount = 18,1579; qualityBinCount = 19,2098; qualityBinCount = 20,772; qualityBinCount = 21,2178; qualityBinCount = 22,1636; qualityBinCount = 23,2600; qualityBinCount = 24,1440; qualityBinCount = 25,2219; qualityBinCount = 26,1610; qualityBinCount = 27,2104; qualityBinCount = 28,2207; qualityBinCount = 29,1293; qualityBinCount = 30,2336; qualityBinCount = 31,1224; qualityBinCount = 32,2740; qualityBinCount = 33,2308; qualityBinCount = 34,757; qualityBinCount = 35,3826; qualityBinCount = 36,671; qualityBinCount = 37,9256; qualityBinCount = 38,1076; qualityBinCount = 39,2834; qualityBinCount = 40,6782; } region = 4; key = TCA; numberReads = 71065; totalBases = 16993096; averageLength = 239.120,57.958; averageQuality = 37.458,5.414; lengthHistogram { lengthCount = 42,1; lengthCount = 44,1; lengthCount = 45,1; lengthCount = 46,1; lengthCount = 47,1; lengthCount = 48,1; lengthCount = 49,3; lengthCount = 50,13; lengthCount = 51,19; lengthCount = 52,95; lengthCount = 53,217; lengthCount = 54,46; lengthCount = 55,33; lengthCount = 56,22; lengthCount = 57,30; lengthCount = 58,27; lengthCount = 59,21; lengthCount = 60,36; lengthCount = 61,130; lengthCount = 62,245; lengthCount = 63,349; lengthCount = 64,125; lengthCount = 65,512; lengthCount = 66,33; lengthCount = 67,27; lengthCount = 68,47; lengthCount = 69,47; lengthCount = 70,102; lengthCount = 71,16; lengthCount = 72,27; lengthCount = 73,39; lengthCount = 74,81; lengthCount = 75,50; lengthCount = 76,296; lengthCount = 77,19; lengthCount = 78,43; lengthCount = 79,32; 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lengthCount = 461,1; lengthCount = 462,2; lengthCount = 463,7; lengthCount = 464,9; lengthCount = 465,2; lengthCount = 466,3; lengthCount = 467,2; lengthCount = 468,4; lengthCount = 470,1; lengthCount = 471,6; lengthCount = 472,1; lengthCount = 473,4; lengthCount = 474,6; lengthCount = 475,7; lengthCount = 476,7; lengthCount = 477,8; lengthCount = 478,5; lengthCount = 479,6; lengthCount = 480,2; lengthCount = 481,3; lengthCount = 482,4; lengthCount = 483,3; lengthCount = 485,6; lengthCount = 486,4; lengthCount = 488,4; lengthCount = 489,2; lengthCount = 490,3; lengthCount = 491,2; lengthCount = 492,2; lengthCount = 493,7; lengthCount = 494,6; lengthCount = 495,10; lengthCount = 496,5; lengthCount = 497,1; lengthCount = 498,5; lengthCount = 499,2; lengthCount = 500,5; lengthCount = 501,6; lengthCount = 502,5; lengthCount = 503,13; lengthCount = 504,7; lengthCount = 505,8; lengthCount = 506,4; lengthCount = 507,4; lengthCount = 508,2; lengthCount = 509,3; lengthCount = 510,3; lengthCount = 511,1; lengthCount = 513,7; lengthCount = 514,1; lengthCount = 516,4; lengthCount = 517,4; lengthCount = 519,7; lengthCount = 520,9; lengthCount = 521,6; lengthCount = 522,3; lengthCount = 523,3; lengthCount = 524,2; lengthCount = 525,3; lengthCount = 526,3; lengthCount = 528,4; lengthCount = 529,2; lengthCount = 530,3; lengthCount = 531,2; lengthCount = 532,1; lengthCount = 533,1; lengthCount = 534,1; lengthCount = 535,2; lengthCount = 538,3; lengthCount = 540,1; lengthCount = 541,2; lengthCount = 545,1; lengthCount = 547,1; lengthCount = 548,2; lengthCount = 549,2; lengthCount = 552,2; lengthCount = 553,1; lengthCount = 557,1; lengthCount = 558,1; lengthCount = 560,1; lengthCount = 561,2; lengthCount = 562,2; lengthCount = 567,1; lengthCount = 588,1; } qualityHistogram { qualityBinCount = 0,1660; qualityBinCount = 7,4; qualityBinCount = 8,75; qualityBinCount = 9,175; qualityBinCount = 10,784; qualityBinCount = 11,27541; qualityBinCount = 12,43547; qualityBinCount = 13,26124; qualityBinCount = 14,54705; qualityBinCount = 15,25542; qualityBinCount = 16,58774; qualityBinCount = 17,50069; qualityBinCount = 18,43226; qualityBinCount = 19,83999; qualityBinCount = 20,70588; qualityBinCount = 21,109469; qualityBinCount = 22,73712; qualityBinCount = 23,78638; qualityBinCount = 24,81425; qualityBinCount = 25,92649; qualityBinCount = 26,200684; qualityBinCount = 27,113204; qualityBinCount = 28,154383; qualityBinCount = 29,122609; qualityBinCount = 30,335327; qualityBinCount = 31,150787; qualityBinCount = 32,153709; qualityBinCount = 33,268029; qualityBinCount = 34,591968; qualityBinCount = 35,237613; qualityBinCount = 36,260671; qualityBinCount = 37,187431; qualityBinCount = 38,368202; qualityBinCount = 39,1814335; qualityBinCount = 40,11111438; } } regionKey { region = 5; key = ATG; numberReads = 1285; totalBases = 668990; averageLength = 520.615,30.815; averageQuality = 33.750,7.694; lengthHistogram { lengthCount = 48,1; lengthCount = 180,1; lengthCount = 181,1; lengthCount = 189,2; lengthCount = 255,1; lengthCount = 482,2; lengthCount = 484,4; lengthCount = 485,2; lengthCount = 486,2; lengthCount = 487,8; lengthCount = 488,46; lengthCount = 489,58; lengthCount = 490,81; lengthCount = 491,53; lengthCount = 492,23; lengthCount = 493,10; lengthCount = 494,6; lengthCount = 495,2; lengthCount = 496,3; lengthCount = 497,2; lengthCount = 498,1; lengthCount = 500,1; lengthCount = 501,1; lengthCount = 502,1; lengthCount = 513,1; lengthCount = 520,2; lengthCount = 521,16; lengthCount = 522,64; lengthCount = 523,140; lengthCount = 524,82; lengthCount = 525,39; lengthCount = 526,18; lengthCount = 527,4; lengthCount = 528,5; lengthCount = 529,4; lengthCount = 530,3; lengthCount = 531,2; lengthCount = 532,3; lengthCount = 533,2; lengthCount = 534,9; lengthCount = 535,13; lengthCount = 536,49; lengthCount = 537,121; lengthCount = 538,213; lengthCount = 539,115; lengthCount = 540,36; lengthCount = 541,19; lengthCount = 542,3; lengthCount = 543,3; lengthCount = 545,2; lengthCount = 546,1; lengthCount = 547,1; lengthCount = 552,1; lengthCount = 554,2; } qualityHistogram { qualityBinCount = 0,84; qualityBinCount = 11,4783; qualityBinCount = 12,3781; qualityBinCount = 13,4085; qualityBinCount = 14,2304; qualityBinCount = 15,5660; qualityBinCount = 16,4370; qualityBinCount = 17,7907; qualityBinCount = 18,6591; qualityBinCount = 19,10097; qualityBinCount = 20,5435; qualityBinCount = 21,10338; qualityBinCount = 22,7429; qualityBinCount = 23,15648; qualityBinCount = 24,8838; qualityBinCount = 25,19360; qualityBinCount = 26,12747; qualityBinCount = 27,17705; qualityBinCount = 28,16440; qualityBinCount = 29,16136; qualityBinCount = 30,19278; qualityBinCount = 31,11351; qualityBinCount = 32,21533; qualityBinCount = 33,21183; qualityBinCount = 34,9060; qualityBinCount = 35,26804; qualityBinCount = 36,15124; qualityBinCount = 37,30840; qualityBinCount = 38,27815; qualityBinCount = 39,45376; qualityBinCount = 40,260888; } region = 5; key = CAT; numberReads = 181; totalBases = 92642; averageLength = 511.834,36.379; averageQuality = 27.691,9.184; lengthHistogram { lengthCount = 93,1; lengthCount = 336,1; lengthCount = 492,1; lengthCount = 495,2; lengthCount = 498,3; lengthCount = 499,7; lengthCount = 500,2; lengthCount = 501,4; lengthCount = 502,13; lengthCount = 503,8; lengthCount = 504,9; lengthCount = 505,6; lengthCount = 506,14; lengthCount = 507,6; lengthCount = 508,9; lengthCount = 509,8; lengthCount = 510,4; lengthCount = 511,4; lengthCount = 512,1; lengthCount = 514,1; lengthCount = 515,4; lengthCount = 519,2; lengthCount = 520,1; lengthCount = 521,1; lengthCount = 522,2; lengthCount = 523,1; lengthCount = 524,2; lengthCount = 525,8; lengthCount = 526,4; lengthCount = 527,6; lengthCount = 528,5; lengthCount = 529,8; lengthCount = 530,2; lengthCount = 531,5; lengthCount = 532,4; lengthCount = 533,2; lengthCount = 534,5; lengthCount = 535,5; lengthCount = 536,1; lengthCount = 537,2; lengthCount = 538,1; lengthCount = 539,1; lengthCount = 540,1; lengthCount = 541,2; lengthCount = 544,1; lengthCount = 545,1; } qualityHistogram { qualityBinCount = 0,40; qualityBinCount = 8,10; qualityBinCount = 9,9; qualityBinCount = 11,4932; qualityBinCount = 12,1469; qualityBinCount = 13,2702; qualityBinCount = 14,1022; qualityBinCount = 15,2974; qualityBinCount = 16,1443; qualityBinCount = 17,2816; qualityBinCount = 18,2506; qualityBinCount = 19,2733; qualityBinCount = 20,898; qualityBinCount = 21,2632; qualityBinCount = 22,2228; qualityBinCount = 23,3599; qualityBinCount = 24,2341; qualityBinCount = 25,2865; qualityBinCount = 26,2270; qualityBinCount = 27,3279; qualityBinCount = 28,3283; qualityBinCount = 29,1906; qualityBinCount = 30,3431; qualityBinCount = 31,1697; qualityBinCount = 32,3859; qualityBinCount = 33,3289; qualityBinCount = 34,1160; qualityBinCount = 35,5046; qualityBinCount = 36,717; qualityBinCount = 37,12883; qualityBinCount = 38,1240; qualityBinCount = 39,4084; qualityBinCount = 40,7279; } region = 5; key = TCA; numberReads = 70918; totalBases = 16980570; averageLength = 239.439,57.693; averageQuality = 37.367,5.499; lengthHistogram { lengthCount = 46,1; lengthCount = 48,1; lengthCount = 50,10; lengthCount = 51,33; lengthCount = 52,160; lengthCount = 53,251; lengthCount = 54,34; lengthCount = 55,27; lengthCount = 56,29; lengthCount = 57,29; lengthCount = 58,47; lengthCount = 59,27; lengthCount = 60,65; lengthCount = 61,149; lengthCount = 62,175; lengthCount = 63,356; lengthCount = 64,56; lengthCount = 65,382; lengthCount = 66,23; lengthCount = 67,20; lengthCount = 68,26; lengthCount = 69,55; lengthCount = 70,74; lengthCount = 71,20; lengthCount = 72,19; lengthCount = 73,41; lengthCount = 74,88; lengthCount = 75,47; lengthCount = 76,450; lengthCount = 77,27; lengthCount = 78,39; lengthCount = 79,13; lengthCount = 80,28; lengthCount = 81,16; lengthCount = 82,19; lengthCount = 83,36; lengthCount = 84,12; lengthCount = 85,8; lengthCount = 86,37; lengthCount = 87,206; lengthCount = 88,51; lengthCount = 89,529; lengthCount = 90,76; lengthCount = 91,33; lengthCount = 92,26; 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lengthCount = 521,3; lengthCount = 522,2; lengthCount = 523,2; lengthCount = 524,1; lengthCount = 526,2; lengthCount = 527,1; lengthCount = 528,3; lengthCount = 529,1; lengthCount = 530,1; lengthCount = 531,3; lengthCount = 532,3; lengthCount = 533,4; lengthCount = 535,1; lengthCount = 537,3; lengthCount = 538,2; lengthCount = 539,2; lengthCount = 540,1; lengthCount = 541,1; lengthCount = 542,2; lengthCount = 543,1; lengthCount = 546,1; lengthCount = 547,1; lengthCount = 548,1; lengthCount = 549,1; lengthCount = 550,1; lengthCount = 551,1; lengthCount = 552,1; lengthCount = 554,1; lengthCount = 561,1; lengthCount = 562,1; lengthCount = 568,1; } qualityHistogram { qualityBinCount = 0,1878; qualityBinCount = 7,9; qualityBinCount = 8,94; qualityBinCount = 9,185; qualityBinCount = 10,654; qualityBinCount = 11,29139; qualityBinCount = 12,47533; qualityBinCount = 13,28976; qualityBinCount = 14,57798; qualityBinCount = 15,25252; qualityBinCount = 16,60617; qualityBinCount = 17,50109; qualityBinCount = 18,44673; qualityBinCount = 19,87456; qualityBinCount = 20,74025; qualityBinCount = 21,108770; qualityBinCount = 22,76505; qualityBinCount = 23,85744; qualityBinCount = 24,84764; qualityBinCount = 25,96451; qualityBinCount = 26,200261; qualityBinCount = 27,120529; qualityBinCount = 28,163670; qualityBinCount = 29,125628; qualityBinCount = 30,338503; qualityBinCount = 31,154917; qualityBinCount = 32,164393; qualityBinCount = 33,284681; qualityBinCount = 34,575530; qualityBinCount = 35,256283; qualityBinCount = 36,275699; qualityBinCount = 37,216706; qualityBinCount = 38,403939; qualityBinCount = 39,1803815; qualityBinCount = 40,10935384; } } regionKey { region = 6; key = ATG; numberReads = 1423; totalBases = 741152; averageLength = 520.838,25.611; averageQuality = 34.246,7.345; lengthHistogram { lengthCount = 97,1; lengthCount = 196,1; lengthCount = 239,1; lengthCount = 467,1; lengthCount = 478,1; lengthCount = 482,1; lengthCount = 483,3; lengthCount = 484,15; lengthCount = 485,4; lengthCount = 486,5; lengthCount = 487,27; lengthCount = 488,107; lengthCount = 489,79; lengthCount = 490,87; lengthCount = 491,12; lengthCount = 492,2; lengthCount = 493,2; lengthCount = 494,3; lengthCount = 495,1; lengthCount = 498,1; lengthCount = 504,1; lengthCount = 514,1; lengthCount = 515,1; lengthCount = 518,1; lengthCount = 519,4; lengthCount = 520,10; lengthCount = 521,28; lengthCount = 522,69; lengthCount = 523,139; lengthCount = 524,83; lengthCount = 525,31; lengthCount = 526,11; lengthCount = 527,4; lengthCount = 528,1; lengthCount = 529,1; lengthCount = 530,1; lengthCount = 531,3; lengthCount = 532,5; lengthCount = 533,3; lengthCount = 534,8; lengthCount = 535,16; lengthCount = 536,61; lengthCount = 537,197; lengthCount = 538,232; lengthCount = 539,103; lengthCount = 540,29; lengthCount = 541,11; lengthCount = 542,6; lengthCount = 543,4; lengthCount = 544,3; lengthCount = 545,1; lengthCount = 559,1; } qualityHistogram { qualityBinCount = 0,64; qualityBinCount = 11,3698; qualityBinCount = 12,3091; qualityBinCount = 13,3532; qualityBinCount = 14,2310; qualityBinCount = 15,4781; qualityBinCount = 16,4176; qualityBinCount = 17,7048; qualityBinCount = 18,5888; qualityBinCount = 19,9381; qualityBinCount = 20,6026; qualityBinCount = 21,10044; qualityBinCount = 22,7279; qualityBinCount = 23,15364; qualityBinCount = 24,9025; qualityBinCount = 25,21220; qualityBinCount = 26,13637; qualityBinCount = 27,19565; qualityBinCount = 28,18954; qualityBinCount = 29,19146; qualityBinCount = 30,22149; qualityBinCount = 31,11825; qualityBinCount = 32,26012; qualityBinCount = 33,22426; qualityBinCount = 34,10581; qualityBinCount = 35,27482; qualityBinCount = 36,17420; qualityBinCount = 37,25236; qualityBinCount = 38,33739; qualityBinCount = 39,47781; qualityBinCount = 40,312272; } region = 6; key = CAT; numberReads = 321; totalBases = 164041; averageLength = 511.031,41.200; averageQuality = 27.632,9.119; lengthHistogram { lengthCount = 66,1; lengthCount = 120,1; lengthCount = 149,1; lengthCount = 468,1; lengthCount = 479,1; lengthCount = 494,1; lengthCount = 495,1; lengthCount = 496,2; lengthCount = 497,4; lengthCount = 498,5; lengthCount = 499,10; lengthCount = 500,13; lengthCount = 501,17; lengthCount = 502,20; lengthCount = 503,15; lengthCount = 504,17; lengthCount = 505,17; lengthCount = 506,11; lengthCount = 507,9; lengthCount = 508,5; lengthCount = 509,3; lengthCount = 510,4; lengthCount = 512,2; lengthCount = 513,1; lengthCount = 514,2; lengthCount = 515,2; lengthCount = 516,3; lengthCount = 517,4; lengthCount = 518,8; lengthCount = 519,7; lengthCount = 520,8; lengthCount = 521,8; lengthCount = 522,3; lengthCount = 523,10; lengthCount = 524,8; lengthCount = 525,9; lengthCount = 526,7; lengthCount = 527,6; lengthCount = 528,8; lengthCount = 529,8; lengthCount = 530,6; lengthCount = 531,11; lengthCount = 532,10; lengthCount = 533,8; lengthCount = 534,5; lengthCount = 535,2; lengthCount = 536,3; lengthCount = 537,3; lengthCount = 538,2; lengthCount = 539,1; lengthCount = 540,1; lengthCount = 541,1; lengthCount = 542,1; lengthCount = 544,1; lengthCount = 545,1; lengthCount = 547,1; lengthCount = 552,1; } qualityHistogram { qualityBinCount = 0,73; qualityBinCount = 8,13; qualityBinCount = 9,28; qualityBinCount = 11,8260; qualityBinCount = 12,2796; qualityBinCount = 13,4414; qualityBinCount = 14,1814; qualityBinCount = 15,5327; qualityBinCount = 16,2583; qualityBinCount = 17,5136; qualityBinCount = 18,4317; qualityBinCount = 19,5443; qualityBinCount = 20,1868; qualityBinCount = 21,4758; qualityBinCount = 22,3919; qualityBinCount = 23,6447; qualityBinCount = 24,4076; qualityBinCount = 25,5087; qualityBinCount = 26,4181; qualityBinCount = 27,5766; qualityBinCount = 28,5760; qualityBinCount = 29,3495; qualityBinCount = 30,6195; qualityBinCount = 31,3114; qualityBinCount = 32,6827; qualityBinCount = 33,5944; qualityBinCount = 34,2020; qualityBinCount = 35,9107; qualityBinCount = 36,1196; qualityBinCount = 37,22406; qualityBinCount = 38,2061; qualityBinCount = 39,7428; qualityBinCount = 40,12182; } region = 6; key = TCA; numberReads = 89718; totalBases = 21693922; averageLength = 241.801,53.709; averageQuality = 37.029,5.759; lengthHistogram { lengthCount = 42,1; lengthCount = 44,1; lengthCount = 45,1; lengthCount = 46,2; lengthCount = 47,1; lengthCount = 48,7; lengthCount = 49,12; lengthCount = 50,35; lengthCount = 51,58; lengthCount = 52,123; lengthCount = 53,343; lengthCount = 54,59; lengthCount = 55,33; lengthCount = 56,29; lengthCount = 57,26; lengthCount = 58,26; lengthCount = 59,18; lengthCount = 60,46; lengthCount = 61,124; lengthCount = 62,185; lengthCount = 63,378; lengthCount = 64,198; lengthCount = 65,349; lengthCount = 66,25; lengthCount = 67,24; lengthCount = 68,25; lengthCount = 69,36; lengthCount = 70,78; lengthCount = 71,20; lengthCount = 72,26; lengthCount = 73,39; lengthCount = 74,77; lengthCount = 75,54; lengthCount = 76,407; lengthCount = 77,16; lengthCount = 78,49; lengthCount = 79,23; lengthCount = 80,18; lengthCount = 81,19; lengthCount = 82,20; lengthCount = 83,27; 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lengthCount = 520,3; lengthCount = 521,2; lengthCount = 522,3; lengthCount = 523,3; lengthCount = 524,3; lengthCount = 525,6; lengthCount = 526,4; lengthCount = 527,3; lengthCount = 528,4; lengthCount = 529,1; lengthCount = 530,1; lengthCount = 531,1; lengthCount = 532,2; lengthCount = 533,3; lengthCount = 534,6; lengthCount = 537,1; lengthCount = 538,1; lengthCount = 540,1; lengthCount = 541,1; lengthCount = 543,1; lengthCount = 544,1; lengthCount = 547,1; lengthCount = 550,1; lengthCount = 555,1; lengthCount = 557,1; lengthCount = 558,1; lengthCount = 610,1; } qualityHistogram { qualityBinCount = 0,1427; qualityBinCount = 7,7; qualityBinCount = 8,89; qualityBinCount = 9,178; qualityBinCount = 10,579; qualityBinCount = 11,42373; qualityBinCount = 12,62901; qualityBinCount = 13,36741; qualityBinCount = 14,84714; qualityBinCount = 15,35462; qualityBinCount = 16,88985; qualityBinCount = 17,71904; qualityBinCount = 18,64490; qualityBinCount = 19,130427; qualityBinCount = 20,109057; qualityBinCount = 21,158595; qualityBinCount = 22,113150; qualityBinCount = 23,128936; qualityBinCount = 24,123058; qualityBinCount = 25,152688; qualityBinCount = 26,285549; qualityBinCount = 27,183501; qualityBinCount = 28,248029; qualityBinCount = 29,187534; qualityBinCount = 30,461872; qualityBinCount = 31,229669; qualityBinCount = 32,252430; qualityBinCount = 33,436911; qualityBinCount = 34,731457; qualityBinCount = 35,392276; qualityBinCount = 36,401573; qualityBinCount = 37,332087; qualityBinCount = 38,657483; qualityBinCount = 39,2218272; qualityBinCount = 40,13269518; } } regionKey { region = 7; key = ATG; numberReads = 940; totalBases = 490108; averageLength = 521.391,27.099; averageQuality = 34.050,7.734; lengthHistogram { lengthCount = 66,1; lengthCount = 155,1; lengthCount = 482,1; lengthCount = 483,2; lengthCount = 484,10; lengthCount = 485,1; lengthCount = 486,6; lengthCount = 487,21; lengthCount = 488,56; lengthCount = 489,40; lengthCount = 490,25; lengthCount = 491,19; lengthCount = 492,9; lengthCount = 493,6; 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qualityBinCount = 16,3186; qualityBinCount = 17,5750; qualityBinCount = 18,4888; qualityBinCount = 19,7162; qualityBinCount = 20,3909; qualityBinCount = 21,6903; qualityBinCount = 22,5339; qualityBinCount = 23,10603; qualityBinCount = 24,5842; qualityBinCount = 25,12964; qualityBinCount = 26,8563; qualityBinCount = 27,11333; qualityBinCount = 28,10847; qualityBinCount = 29,10288; qualityBinCount = 30,12500; qualityBinCount = 31,7803; qualityBinCount = 32,14434; qualityBinCount = 33,13616; qualityBinCount = 34,6484; qualityBinCount = 35,18507; qualityBinCount = 36,11151; qualityBinCount = 37,23692; qualityBinCount = 38,19282; qualityBinCount = 39,34792; qualityBinCount = 40,204073; } region = 7; key = CAT; numberReads = 167; totalBases = 85421; averageLength = 511.503,30.193; averageQuality = 27.282,9.226; lengthHistogram { lengthCount = 260,1; lengthCount = 347,1; lengthCount = 365,1; lengthCount = 431,1; lengthCount = 470,1; lengthCount = 495,1; lengthCount = 498,3; lengthCount = 499,2; 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qualityBinCount = 14,1039; qualityBinCount = 15,2789; qualityBinCount = 16,1361; qualityBinCount = 17,2709; qualityBinCount = 18,2297; qualityBinCount = 19,2752; qualityBinCount = 20,996; qualityBinCount = 21,2451; qualityBinCount = 22,2038; qualityBinCount = 23,3409; qualityBinCount = 24,2107; qualityBinCount = 25,2686; qualityBinCount = 26,1906; qualityBinCount = 27,2996; qualityBinCount = 28,2951; qualityBinCount = 29,1789; qualityBinCount = 30,3168; qualityBinCount = 31,1683; qualityBinCount = 32,3639; qualityBinCount = 33,2931; qualityBinCount = 34,998; qualityBinCount = 35,4640; qualityBinCount = 36,664; qualityBinCount = 37,11993; qualityBinCount = 38,1004; qualityBinCount = 39,3399; qualityBinCount = 40,5818; } region = 7; key = TCA; numberReads = 84073; totalBases = 20101795; averageLength = 239.099,56.843; averageQuality = 37.276,5.642; lengthHistogram { lengthCount = 43,1; lengthCount = 45,2; lengthCount = 46,1; lengthCount = 48,3; lengthCount = 49,5; lengthCount = 50,10; lengthCount = 51,27; 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